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A new potentiometric sensor for the determination of α-amylase activity


Sakač, Nikola; Sak-Bosnar, Milan; Horvat, Marija; Madunić-Čačić, Dubravka; Szechenyi, Aleksandar; Kovacs, Barna
A new potentiometric sensor for the determination of α-amylase activity // Talanta, 83 (2011), 5; 1606-1612. doi:10.1016/j.talanta.2010.11.053 (članak, znanstveni)


Naslov
A new potentiometric sensor for the determination of α-amylase activity

Autori
Sakač, Nikola ; Sak-Bosnar, Milan ; Horvat, Marija ; Madunić-Čačić, Dubravka ; Szechenyi, Aleksandar ; Kovacs, Barna

Izvornik
Talanta (0039-9140) 83 (2011), 5; 1606-1612

Vrsta, podvrsta i kategorija rada
Radovi u časopisima, članak, znanstveni

Ključne riječi
Α-amylase; potentiometric sensor; starch; triiodide

Sažetak
A platinum redox sensor for the direct potentiometric determination of α-amylase concentration has been described. The sensor measured the amount of triiodide released from a starch-triiodide complex, which was correlated with the α-amylase activity after biocatalytic starch degradation. The composition and stability of the potassium triiodide solution was optimized. The starch-triiodide complex was characterized potentiometrically at variable starch and triiodide concentrations. The response mechanism of the platinum redox sensor towards α-amylase was proposed and the appropriate theoretical model was elaborated. The results obtained using the redox sensor exhibited satisfactory accuracy and precision and good agreement with a standard spectrophotometric method and high-sensitive fully automated descret analyser method. The sensor was tested on pure α-amylase (EC 3.2.1.1, Fluka, Switzerland), industrial granulated α-amylase Duramyl 120 T and an industrial cogranulate of protease and α-amylase Everlase/Duramyl 8.0 T/60 T. The detection limit was found to be 1.944 mU for α-amylase in the range of 0-0.54 U (0-15 μg), 0.030 mKNU for Duramyl 120 T in the range of 0-9.6 mKNU (0-80 μg) and 0.032 mKNU for Everlase/Duramyl 8.0 T/60 T in the range of 0-9.24 mKNU (0-140 μg).

Projekt / tema
Kemijski senzori za primjenu u biomedicini, hrani i zaštiti okoliša (291-0580000-0169)

Izvorni jezik
Engleski

Indeksirano u
Current Contents , Web of Science Core Collection

Znanstvena područja
Kemija, Kemijsko inženjerstvo, Biotehnologija